How To Clean The Check Valve On Pump P-900

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FAQ

I desire to know the DIMENSIONS and WEIGHT of my system.

I want to know the DIMENSIONS and WEIGHT of my system.

System	Superlative (mm)	Footprint (mm 10 mm)	Weight (kg)	Menstruum charge per unit (ml/min)	Pressure limit (MPa)
ÄKTA avant 25	660	860 x 710	116	0.001-25	twenty
ÄKTA avant 150	660	860 x 710	116	0.001-150 (normal range) 0.001-300 (column packing flow)	5
ÄKTA pure	630	535 x 470	upwardly to 53 kg	0.001 to 25 (upward to 50 during column packing)	20
ÄKTA pure 150	630	535 x 470	upwardly to 53 kg	0.01 to 150 (upwardly to 300 during column packing)	5
ÄKTAexplorer 10	620	500 x 460	75	0.001-x	25
ÄKTAexplorer 100	620	500 x 460	75	0.01-100	10
ÄKTAFPLC	470	380 x 480	l	0.05-20	v
ÄKTAmicro	610	480 10 450	55	0.001-two	35
ÄKTApilot	900	750 x 540	114	4-400 (full gradients) 4-800 (limited gradients)	2
ÄKTAprime plus	530	400 10 450	13	0.1-50	i
ÄKTApurifier x	620	500 x 460	75	0.001-10	25
ÄKTApurifier 100	620	500 x 460	75	0.01-100	10
ÄKTAxpress	660	490 x 250	30	0.1-65	iii
Ettan LC	610	480 x 450	55	0.001-two	35
Ettan MDLC	710	700 x 640	105	0.001-two	35
Ettan microLC	1150	650 x 500	77	0.001-ii	35
Ettan nanoLC	1150	650 x 500	77	0.001-2	35

Which EMPTY COLUMNS are recommended for my system?

Which EMPTY COLUMNS are recommended for my system?

ÄKTAbasic 100, ÄKTAexplorer 100, ÄKTAexplorer 100 Air, ÄKTApurifier 100
and ÄKTApurifier 100 UPC

Please consider that the maximum menstruum rate for these systems is 100 ml/min and
maximum pressure is 10 MPa.

Columns
BPG 100
C ten
C 16
C 26
FineLINE Pilot 35
HR xvi, High Resolution Cavalcade
Chiliad nine
PD-10, Dispensable Column
SR 25, Solvent Resistant Cavalcade
Tricorn 5, High Performance Column
Tricorn ten, High Performance Column
XK sixteen
XK 26
XK 50
HiScale 16
HiScale 26
HiScale fifty

Why should I accept REGULAR, PLANNED MAINTENANCE on my system?

Why should I have REGULAR, PLANNED MAINTENANCE on my system?

With the pressure on producing sample or results, the condition of your ÄKTAdesign or Ettan system is critical and regular servicing will hateful you tin depend on your system to perform as expected. Planned maintenance tin be part of a service agreement, scheduled to service your system before it is in need of attention. We tin can help you design a schedule and routine to allow you to maintain your organisation, please contact your local Cytiva service representative.

So what can you look from a planned maintenance visit from Cytiva service representative?

• Thorough inspection and cleaning of organization components
• Update of system firmware to ensure full compatibility of your system and UNICORN software
• Replacement of damaged or corroded seals, valve springs and solenoids
• Replacement of items that are reaching the end of their expected life – preventing futurity breakdowns
• Advice and guidance on proper daily utilise, cleaning and care of your organisation
• All piece of work is documented and reported to aid brand any regulatory audits easier.

A complete overhaul, once a year, ensures that your musical instrument is running at peak performance so y'all can be confident of your scientific results. In addition, wear and tear on systems under constant use by multiple end users is minimized, giving the arrangement a longer life and improve value for coin.

To discover out more about service possibilities contact your local Cytiva service representative.

I desire to know the FLOW and PRESSURE RANGE of my system?

I want to know the FLOW and PRESSURE RANGE of my arrangement.

Organization	Height (mm)	Footprint (mm x mm)	Weight (kg)	Flow rate (ml/min)	Force per unit area limit (MPa)
ÄKTA avant 25	660	860 ten 710	116	0.001-25	20
ÄKTA avant 150	660	860 ten 710	116	0.001-150 (normal range) 0.001-300 (column packing menstruation)	5
ÄKTA pure	630	535 x 470	up to 53 kg	0.001 to 25 (up to 50 during column packing)	20
ÄKTA pure 150	630	535 10 470	up to 53 kg	0.01 to 25 (upwardly to 300 during column packing)	5
ÄKTAexplorer x	620	500 ten 460	75	0.001-ten	25
ÄKTAexplorer 100	620	500 x 460	75	0.01-100	10
ÄKTAFPLC	470	380 ten 480	50	0.05-20	5
ÄKTAmicro	610	480 x 450	55	0.001-2	35
ÄKTApilot	900	750 x 540	114	iv-400 (full gradients) 4-800 (express gradients)	2
ÄKTAprime plus	530	400 x 450	13	0.1-50	1
ÄKTApurifier ten	620	500 x 460	75	0.001-ten	25
ÄKTApurifier 100	620	500 x 460	75	0.01-100	10
ÄKTAxpress	660	490 x 250	30	0.i-65	3
Ettan LC	610	480 10 450	55	0.001-ii	35
Ettan MDLC	710	700 10 640	105	0.001-2	35
Ettan microLC	1150	650 x 500	77	0.001-2	35
Ettan nanoLC	1150	650 x 500	77	0.001-2	35

How tin I Exam THE PERFORMANCE of my system?

How can I Examination THE PERFORMANCE of my ÄKTA arrangement?

The performance of all UNICORN controlled ÄKTA systems can be checked by using the installation test that is part of each systems strategy. The installation test checks the function of the liquid commitment and UV monitor systems and tin can be performed at whatsoever time to check the condition of the system.

Correct slope germination is tested by producing a linear and step gradient of acetone and water. The UV monitoring arrangement is tested by monitoring the absorbance of the acetone at 265, 254 and 280 nm.

Each installation exam is designed to be run with the system nether pressure level equally this ensures the efficient opening and endmost of the check valves. For systems based on the pumps P-901 and P-903 this pressure is provided by internal restriction in the pumps.

For sanitary systems such as the ÄKTApilot™ the required pressure is provided by the cavalcade. Therefore, when performing the ÄKTApilot installation exam you must provide pressure to the system, either by using a standard FR-902 flow restrictor or past fitting a length of capillary tubing that gives >0.1 MPa of back pressure.

What DOCUMENTS are supplied with my system?

What DOCUMENTS are supplied with my system?

ÄKTApurifier
ÄKTApurifier User Guide
ÄKTApurifier Getting started
Purification Method Handbook
Optional Configurations ÄKTApuirifier User Manual
Pump P-900 User Transmission
Short Instructions P-900
Monitor UV-900 User Manual
Brusk Instructions UV-900
Monitor UPC-900 User Manual
Valve INV-907 Instructions
Mixer Yard-925 Instructions

Using UNICORN with Windows XP Service Pack 2
Getting Started with UNICORN
UNICORN User Reference Transmission affiliate i-xi
UNICORN User Reference Manual chapter 12-fifteen
UNICORN Administration and Technical Transmission
System certificate

Module certificate for
Pump P-900
Monitor UV-900 (ÄKTApurifier)
Monitor UPC-900 (ÄKTApurifier UPC)
Valve INV-907

What kind of ORGANIC SOLVENT tin I utilise with my ÄKTAdesign arrangement?

What kind of ORGANIC SOLVENT can I use with my ÄKTAdesign arrangement?

ÄKTAbasic, ÄKTAexplorer, ÄKTAFPLC, ÄKTAmicro, ÄKTAprime, ÄKTAprime plus, ÄKTApurifier, Ettan LC, Ettan microLC, Ettan nanoLC and Ettan MDLC

Generally: The wetted parts are resistant to organic solvents and salt buffers commonly used in chromatography of biomolecules, except 100% Ethyl acetate, 100% Hexane and 100% Tetrahydrofuran.

Chemical resistance guide and chemical compatibility
The chemical resistance to some of the most commonly used chemicals in liquid chromatography is indicated in the table below.

The ratings are based on the following assumptions:
1. The synergistic effects of the chemical mixtures have not been taken into account.
2. Room temperature and limited over-pressure level is assumed.

Note: Chemic influences are time and pressure dependent. Unless otherwise stated, all concentrations are 100%.

Chemic	Exposure < 1 day	Exposure up to 2 months	Comments
Acetaldehyde	OK	OK
Acetic acid, < 5%	OK	OK
Acetic acid, 70%	OK	OK
Acetonitrile	OK	Avoid	FFKM, PP and PE swell. PEEK is affected by long term use
Acetone, 10%	OK	Avoid	PVDF is affected by long term apply
Ammonia, xxx%	OK	OK	Silicone is affected past long term use
Ammonium chloride	OK	OK
Ammonium bicarbonate	OK	OK
Ammonium nitrate	OK	OK
Ammonium sulphate	OK	OK
1-Butanol	OK	OK
2-Butanol	OK	OK
Citric acrid	OK	OK
Chloroform	OK	Avoid	ECTFE,CTFE, PP and PE are affected past long term apply
Cyclohexane	OK	OK
Detergents	OK	OK
Dimethyl sulphoxide	Avoid	Avoid	PVDF is afflicted by long term apply
1, four-Dioxane	Avoid	Avoid	ETFE, CTFE, PP, PE and PVDF are affected by long term use
Ethanol	OK	OK
Ethyl acetate	OK	Avert	Silicone non resistant. Force per unit area limit for PEEK decreases.
Ethylene glycol	OK	OK
Formic acrid	OK	OK	Silicone not resistant
Glycerol	OK	OK
Guanidinium hydrochloride	OK	OK
Hexane	OK	Avoid	Silicone non resistant. Pressure limit for PEEK decreases.
Hydrochloric acid, 0.1 One thousand	OK	OK	Silicone not resistant
Hydrochloric acid, > 0.1 M	OK	Avoid	Silicone not resistant. Titanium is affected past long term use
isopropanol	OK	OK
Methanol	OK	OK
Nitric acid, diluted	OK	Avoid	Silicone not resistant
Nitric acid, 30%	Avoid	Avoid	Elgiloy is afflicted by long term use
Phosphoric acrid, x%	OK	Avoid	Titanium and aluminium oxide are afflicted past long term use
Potassium carbonate	OK	OK
Potassium chloride	OK	OK
Pyridine	Avoid	Avoid	ETFE, PP and PE not resistant
Sodium acetate	OK	OK
Sodium bicarbonate	OK	OK
Sodium bisulphate	OK	OK
Sodium borate	OK	OK
Sodium carbonate	OK	OK
Sodium chloride	OK	OK
Sodium hydroxide, 2 One thousand	OK	Avoid	Titanium, PVDF and borosilicate drinking glass are affected by long term apply
Sodium sulphate	OK	OK
Sulphuric acid, diluted	OK	Avoid	PEEK and titanium are affected past long term use
Sulphuric acid, medium concentration	Avoid	Avoid
Tetrachloroethylene	Avoid	Avoid	Silicone, PP and PE are non resistant
Tetrahydrofuran	Avoid	Avoid	Silicone, ETFE, CTFE, PP and PE are non resistant
Toluene	OK	Avoid	Force per unit area limit for PEEK decreases
Trichloroacetic acid, 1%	OK	OK
Trifluoroacetic acid, 1%	OK	OK
Urea	OK	OK
o-Xylene p-Xylene	OK	Avoid	Silicone, PP and PE are affected by long term use

How can I decide the delay book?

How tin can I make up one's mind the delay book?

Three different methods are described below:

Method I - Determining the delay volume of your system by measuring different retention volumes

1) Check that the pump is delivering the correct flow at 1 ml/min. If the measured flow charge per unit differs the retention volumes demand to be corrected.

2) Mount a pocket-sized loop, e.1000. 100 µl, and prepare a solution of 5% acetone in water to employ as sample.

3) Make full the system with clean h2o. Run manually or make your own method. Start the pump at ane ml/min and inject the sample. The measured retention volume is called Volume one.

4) Re-configure your system. Dismount the tubing from the UV flow jail cell and insert a low dead book connector, eastward.g. a female-female Valve connector, in the flow path (replaces the UV menses prison cell). Mount the "tubing end" of the frac outlet into the UV menses jail cell and mountain a waste matter-tubing to the bottom of the UV flow cell.

v) Set Frac size to a very large volume, e.g. 100 ml, so that the valve is in the Frac position during the entire run. Kickoff the pump at 1 ml/min and inject the sample. The measured retention volume is called Volume 2. Delay book = Book ii – Volume ane.

Method II - Determining the delay volume of your system with the assistance of another ÄKTA system

This method is more accurate than the 1 described in a higher place, but demands one more system.

1) Check that the pump on your chosen ÄKTA arrangement is delivering the correct flow at one ml/min. If the measured flow rate differs the retention volumes demand to be corrected.

2) Mount a small loop, due east.thou. 100 µl, and prepare a solution of 2-v% acetone to use as sample.

3) Fill up the organization with clean water. Run manually or make your own method. Start the pump at i ml/min and inject the sample. The measured retention book is called Volume 1.

4) Re-configure your organisation. In east.g. an ÄKTAprime system dismount the tubing from the UV menstruum jail cell to the Fraction collector including the restrictor, the Cond cell and the Frac valve. In the other ÄKTA system, e.one thousand. ÄKTApurifier or ÄKTAexplorer, mountain the "prime tubing kit" between the tubing to the UV flow cell and the UV period cell. To do this a depression dead volume connector, due east.g. a female-female person Valve connector, is needed.

five) In order to have the correct flow path the Frac valve in the "prime tubing kit" must exist activated. This can be achieved be connecting it to the Valve B port of the P-900 pump in the ÄKTApurifier or ÄKTAexplorer system. The valve is activated past setting Pump B inlet to the B2 position.

6) Commencement the pump at 1 ml/min and inject the sample. The measured retention volume is called Volume 2. Delay book = Volume ii – Volume i.

Method Three - Determining the delay volume by balancing eluted water

Manually set the flow path to the direction of the fraction collector. Unscrew the tubing that is connected to inlet of the UV flow cell and insert a luer adaptor instead. Fill a syringe with water and inject h2o into flow cell unless it drops at the outlet of the fraction collector (in which instance you take likely exceeded the pressure in the tubing which might be more than 4 bar, depending on configuration and flow restrictor used). Now fill the syringe with air (at to the lowest degree 20 ml because of compression) and displace the water. Collect eluting water in a small loving cup. Determine the system filibuster volume by balancing the loving cup before and after elution. Repeat two times for calculation of a mean value. Enter the hateful value in "system settings" in UNICORN.

How can I USE my arrangement in COLD ROOMS?

How can I use my system in cold rooms?

Purifying some samples at room temperature can lead to increased levels of degradation; performing your purifications at 4C tin help. All our ÄKTA systems, Ettan LC, Ettan microLC, Ettan nanoLC and fraction collectors are suitable for use in the temperature range of 4-40C.

The calculator systems are not however cold room uniform, and can be damaged by being placed at 4C.

When installing an ÄKTA system in a cold room the computer can be positioned up to 15 1000 away, allowing it to be positioned exterior the cold room. Cold cabinets tin can provide an constructive solution to running your ÂKTA system in the common cold whilst protecting the PC. When moving a organisation to or from a cold room, time must be allowed for the system to adjust to its new temperature. Yous may too detect that y'all need to tighten the connectors on your system slightly to prevent leaks when you bring a system out from the common cold, and loosen them slightly before you put a system into the common cold to prevent pressure build upwards.

Changes in temperature tin also touch on the viscosity of your buffers so go on a close centre on your back pressure.

How tin can I clean my system?

ÄKTApurifier and ÄKTApurifier UPC

Cleaning the system catamenia path
The procedures below draw how to clean the arrangement flow path.

To bypass the column, use a capillary tubing supplied with your system. If the column is to exist left in the menstruation path, brand sure that the maximum allowed menses and pressure for the column are not exceeded. For column cleaning procedures and column storage instructions, please refer to
the pedagogy supplied with the column.

At the terminate of the day
If the organisation will exist used with the aforementioned buffers the side by side day, let the system run isocratic at a low flow rate (approximately 0.02 ml/min). If the system will be used with other buffers the adjacent mean solar day, rinse the pump and the system with distilled water using the PumpWash instruction as follows:

1. Submerge the inlet tubings in distilled h2o.

2. Run the didactics

ÄKTApurifier	ÄKTApurifier UPC
PumpWashPurifier	PumpWashBasic

Leaving the system for a few days
Perform a PumpWashPurifier/PumpWashBasic with distilled water. Echo with
a bacteriostatic solution, i.due east. twenty% ethanol.

Monthly cleaning
Make clean the system every month, or when problems such as ghost peaks occur. Likewise use this procedure to clean the flow path before service or maintenance.

1. Disconnect the column and replace it with bypass tubing.

2. If using a pH electrode, supervene upon information technology with a dummy electrode.

iii. Place all the inlet tubings in 1 Grand NaOH.

four. Manually perform PumpWashPurifier/PumpWashBasic for both inlet tubings.

5. Flush the whole system with one One thousand NaOH for 20 minutes (ane ml/min).

6. Immediately repeat steps 3 and 4 with distilled water to rinse the organization of
NaOH.

Cleaning-in-place
Afterwards repeated separation cycles, contaminating fabric might progressively build up in the system and on the cavalcade. This material may not have been removed by the cleaning step described previously The nature and degree of contamination depends on the sample and the chromatographic weather employed. These should be considered when designing a cleaning process.

HOW LONG should the UV LAMP on my system Last?

How long should the UV LAMP on my system Concluding?

I am getting the error message ‛Fault 71: Warning low light intensity'.

The ÄKTAbasic, ÄKTApurifier, ÄKTAexplorer, ÄKTAmicro and Ettan LC systems contain a
UV-900 monitor, the light source for which is a xenon flash lamp. Xenon lamps
emit a high intensity continuous spectrum of light, your chosen wavelengths are
selected using a monochromator. The lamp is triggered only when needed,
this extends its lifespan, pregnant the average lamp tin withstand many years
of normal use.

The UV monitoring arrangement of the ÄKTAprime, ÄKTAxpress, ÄKTAFPLC, Ettan
microLC, Ettan nanoLC and the Ettan MDLC uses a Zn lamp for monitoring at 214 nm and a Hg lamp
at all other wavelengths. Wavelengths are selected using a ring pass filter. When
the arrangement is operated at room temperature with a wavelength of 254 nm, the boilerplate
Hg lamp lifetime is 7000 hours. In the cold room the average lifetime is reduced to
2000 hours.

I of the most common causes for the mistake message
‛Error 71: WARNING low light intensity' is non lamp failure only a dirty menstruum cell.
Therefore, the first affair that you should do if you get this message is give your
system a skillful make clean. If you are using an ÄKTAFPLC, ÄKTAxpress or ÄKTAprime
this error can besides be caused past incorrect positioning of the lamp. There are two
positions for aligning the Hg lamp with the filter housing, one for 280 nm
(marked past a filled white circle) and one for all other wavelengths
(marked by a white ring). Having the lamp and filter housings incorrectly aligned
can consequence in a low light intensity warning.

I would like to PROGRAMMING UNICORN are there any examples available?

I would like to PROGRAM UNICORN are in that location any examples available?

Please read Method example in UNICORN Users Reference Manual.

Where can I find the latest manuals for UNICORN?

Where can I notice the latest manuals for UNICORN?

We ship the near recent available manual and support documentation at the time
nosotros transport the software to y'all. Retain this transmission as it is the correct one for the version
of software you purchased. Early versions of UNICORN had paper manuals,
merely more than recent versions accept manuals included on CD.

Spare parts

Tubing

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#	Product Name	Product Code	Price
i	PEEK Tubing, 2 m, i.d. 0.v mm, o.d. 1/16"	18111368	61.47 USD	Add to cart Get Quote
ii	PEEK Tubing, 2 m, i.d. 0.75 mm, o.d. 1/sixteen"	18111253	80.72 USD	Add to cart Get Quote
3	PEEK Tubing, 2 m, i.d. one.0 mm, o.d. i/16"	18111583	69.21 USD	Add to cart Get Quote
4	ETFE Tubing, 1.5 yard, i.d. 0.75 mm, o.d. 1/16"	18111974	67.94 USD	Add to cart Go Quote
5	FEP Tubing, i.d. 1/8 in (3/16 in)	18111247	88.threescore USD	Add together to cart Get Quote
6	ETFE Tubing, 2 one thousand, i.d. 0.v mm, o.d. 1/sixteen in	18112096	61.00 USD	Add to cart Get Quote
7	ETFE Tubing, 1.5 yard, i.d. 0.75 mm, o.d. i/16"	18111974	67.94 USD	Add to cart Get Quote

Spare sparts for Sample inlet

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Spare parts, front end view

Figure. ÄKTApurifier UPC 100, front view.

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#	Product Name	Product Code	Price
1	Bespeak Cable, (half-dozen-pin miniDin-Open up)	18111064	321.00 USD	Add to cart Get Quote
ane	Rinsing/draining housing	18111203	90.84 USD	Add together to cart Become Quote
ane	Piston kit 100 ml	18111213	606.00 USD	Add to cart Get Quote
1	Inlet manifold 100 ml	18111225	94.38 USD	Add to cart Get Quote
1	Seal kit 100 ml	18111312	775.00 USD	Add to cart Become Quote
ane	Tubing Kit for Rinsing System	18111332	357.00 USD	Add together to cart Get Quote
1	Pumphead capillaries	18111752	108.93 USD	Add together to cart Get Quote
1	New Purge Kit P-900	18112453	76.56 USD	Add together to cart Get Quote
1	Pumphead complete 100 ml	18112848	ane 867.00 USD	Add to cart Go Quote
one	Bank check valve kit	18112866	1 134.00 USD	Add to cart Get Quote
i	Purge valve	18112887	205.00 USD	Add together to cart Get Quote
1	O-Ring, 1 × iii mm, PFR	19003601	73.60 USD	Add together to cart Get Quote
2	Filter 254 nm	18062001	683.00 USD	Add to cart Get Quote
ii	Filter 280 nm	18062101	662.00 USD	Add together to cart Get Quote
2	Filter 214 nm	18062201	760.00 USD	Add to cart Get Quote
ii	Filter 365 nm	18062401	750.00 USD	Add to cart Get Quote
ii	Filter 405 nm	18062501	679.00 USD	Add together to cart Get Quote
two	Filter 436 nm	18062601	679.00 USD	Add to cart Get Quote
2	Filter 546 nm	18062701	679.00 USD	Add to cart Get Quote
2	Dummy Electrode, round tip	18111103	187.27 USD	Add to cart Become Quote
2	pH Electrode, circular tip	18111126	1 473.00 USD	Add to cart Become Quote
2	pH Menses Jail cell	18111292	1 204.00 USD	Add together to cart Get Quote
2	pH Electrode with Cell and Holder, round tip	18113484	2 280.00 USD	Add to cart Go Quote
ii	Flow cell holder	18305587	345.00 USD	Add to cart Get Quote
two	Hg optics with 254 and 280 nm filters	28404223	five 975.00 USD	Add together to cart Become Quote
2	Zn optics with 214 nm filter	28404224	five 975.00 USD	Add to cart Get Quote
2	Hg lamp and housing consummate	28404225	ane 850.00 USD	Add to cart Get Quote
2	Zn lamp and housing complete	28404226	2 426.00 USD	Add to cart Get Quote
two	Hg lamp	28935493	1 830.00 USD	Add to cart Get Quote
two	Zn lamp	28936572	one 857.00 USD	Add to cart Get Quote
iii	Stop Plug 1/16" Male	18111252	49.lx USD	Add together to cart Become Quote
3	Mixer One thousand-925 for classic ÄKTA	18111889	2 192.00 USD	Add together to cart Become Quote
four	Mixer Yard-925 Mixing Chamber, 0.half dozen ml	18111890	825.00 USD	Add together to cart Become Quote
4	Mixer M-925 Mixing Chamber, 2 ml	18111891	494.00 USD	Add to cart Go Quote
four	Mixer M-925 Mixing Chamber, 5 ml	18111892	555.00 USD	Add together to cart Get Quote
four	Mixer Yard-925 Mixing Chamber, 12 ml	18111893	645.00 USD	Add to cart Get Quote
5	Motor Valve INV-907	18110840	3 946.00 USD	Add to cart Get Quote
5	Repair kit, valve INV-907	18110905	863.00 USD	Add together to cart Get Quote
5	Mounting Subclass for Valves	18110911	29.09 USD	Add to cart Become Quote
5	Injection Kit, INV-907	18111089	527.00 USD	Add together to cart Get Quote
5	Sample loop 100 µl	18111398	157.13 USD	Add to cart Go Quote
5	Sample loop 500 µl	18111399	204.00 USD	Add to cart Get Quote
five	Sample loop 1 ml	18111401	171.12 USD	Add to cart Become Quote
5	Sample Loop 2.0 ml, INV-907	18111402	255.00 USD	Add to cart Go Quote
5	Sample loop, ten µl	18112039	67.94 USD	Add to cart Get Quote
five	Fill Port, INV-907	18112766	223.00 USD	Add to cart Get Quote
five	Sample Loop 5 ml, PEEK	18114053	193.72 USD	Add together to cart Get Quote
6	Short cavalcade holder, for columns 10 to 50 mm o.d.	18111317	178.48 USD	Add to cart Get Quote
6	Long Column Holder, columns 26 mm i.d.	18112632	263.00 USD	Add to cart Get Quote
seven	Electrical conductivity Period Prison cell, 1/16"	18111105	1 967.00 USD	Add together to cart Get Quote
seven	Clamp, electrical conductivity cell	18111114	102.24 USD	Add to cart Get Quote
viii	UV flow cell, 5 mm, for U9-Fifty	18112824	two 166.00 USD	Add to cart Get Quote
9	Flow Restrictor FR-904	18111963	358.00 USD	Add to cart Get Quote
9	Flow Restrictor FR-902	18112135	615.00 USD	Add to cart Get Quote
10	Inlet filter holder kit	11000407	140.nineteen USD	Add together to cart Get Quote
10	Spiral lid kit	11000410	41.97 USD	Add to cart Get Quote
10	Inlet filter set up	11000414	91.66 USD	Add to cart Get Quote
10	Inlet Filter Assembly Kit	18111315	159.60 USD	Add to cart Get Quote
10	Inlet tubing kit, i.d. 2.nine mm, o.d. 3/16 in	18111440	150.97 USD	Add to cart Become Quote
10	Inlet Filter Set	18111442	167.90 USD	Add together to cart Get Quote

Unions, ferrules and terminate plug

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Spare parts not indicated in the view

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#	Production Name	Production Code	Price
1	Cable UniNet, L = 0.xviii m	18110972	119.46 USD	Add to cart Get Quote
2	Cable UniNet, L = 0.iii yard	18110973	131.34 USD	Add to cart Get Quote
3	Cablevision UniNet, L = one.5 m	18111775	213.00 USD	Add to cart Become Quote
four	Cable UniNet, L = three m	18110975	319.00 USD	Add to cart Get Quote
5	Cable UniNet L=15 m	18111774	425.00 USD	Add to cart Become Quote
six	Mains cable, 120 V	19244701	57.98 USD	Add to cart Get Quote
seven	Mains cable 220 V	19244801	89.86 USD	Add to cart Go Quote
8	Betoken Cable, (half-dozen-pin miniDin-Open)	18111064	321.00 USD	Add to cart Get Quote
nine	Online Filter Holder (archetype ÄKTA systems)	18111244	527.00 USD	Add together to cart Get Quote
10	Online filter kit	18102711	120.78 USD	Add to cart Get Quote
11	On-line filter kit	18113023	147.73 USD	Add to cart Get Quote
12	Small Cavalcade Clamp	18114998	40.98 USD	Add to cart Go Quote
xiii	Tubing cutter, for PEEK, EFTE, and FEP tubing i.d. 0.25, 0.5, 0.75, 1 and 1.6 mm	18111246	80.74 USD	Add to cart Get Quote
14	Lab Rod Holder	18111319	398.00 USD	Add to cart Get Quote

Spare parts recommended to go on on site

Seals are considered as consumables and should exist replaced annually or when needed. Please keep a set of each in your stock to forestall long downtime. For code numbers refer to the table below.

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Spare parts, Accessory box delivered with the system

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Unicorn upgrade

ÄKTAbasic, ÄKTAexplorer, ÄKTApurifier

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Menses path schemes

Tubing configuration for loading a Sample loop or multi-loading a Sample loop

Tubing configuration when using sample pump P-960 for loading onto column

Capillary (max. length)	Connector	Connection points
1 PEEK i.d. 0.75 mm x 20 cm (Sample inlet tubing)	Fingertight connector one/16", eighteen-1112-55	Injection valve, port 4
Fingertight connector one/16", 18-1112-55	Sample valve, center port*
2 I.d. 1.0 mm x 50 cm	Fingertight connector 1/16", 18-1112-55	Injection valve, port 3
Fingertight connector i/xvi", xviii-1112-55 Wedlock fingertight female person/M6 male, 18-1112-57	Sample pump, inlet
3 I.d. 1.0 mm x 130 cm	Fingertight connector 1/sixteen", eighteen-1112-55	Injection valve, port five
4 I.d. 1.0 mm ten 10 cm	Fingertight connector 1/16", eighteen-1112-55	Sample pump, outlet
Fingertight connector one/16", 18-1112-55	Menses restrictor, inlet port
five I.d. one.0 mm ten 120 cm	Fingertight connector i/16", eighteen-1112-55	Flow restrictor, outlet port
6 I.d. 1.0 mm 10 32 cm	Fingertight connector 1/xvi", 18-1112-55	Sample valve, inlets S1-S7*
7 PTFE i.d. one.6 mm x 130 cm	Tubing conn. one/viii" o.d. tubing, 18-1121-17 with ferrule for i/8" o.d tubing, eighteen-1121-18. Wedlock 5/16" female person/HPLC male, 18-1142-08	Sample valve, inlet S8*

*Only if a sample valve is installed. Connect stop plugs to non-used ports in the sample valve.

Fingertight connector 1/sixteen" Union fingertight female person/M6 male Union v/16" female/HPLC male person Tubing connector for 1/viii" o.d. tubing with ferrule for 1/eight" o.d. tubing

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ÄKTApurifier 10 UPC and ÄKTApurifier 100 UPC

For information regarding each component, place the cursor over the component of interest.

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Multi-loading a Super loop

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Contrary menstruum valve

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Tubing configuration for loading a Super loop or for multi-loading a Super loop

Tubing configuration when using sample pump P-960 for loading onto column

Capillary (max. length)	Connector	Connection points
1 I.d. ane.0 mm x 130 cm (Sample inlet tubing)	Fingertight connector 1/sixteen", 18-1112-55 Wedlock fingertight female/ M6 male, 18-1112-57	Sample pump, inlet
2 I.d. 1.0 mm x 50 cm	Fingertight connector 1/16", 18-1112-55	Sample pump, outlet
Fingertight connector i/xvi", 18-1112-55	Injection valve, port iii
3 I.d. 1.0 mm x 130 cm	Fingertight connector 1/16", 18-1112-55	Injection valve, port iv
iv I.d. 1.0 mm x ten cm	Fingertight connector one/16", eighteen-1112-55	Injection valve, port v
Fingertight connector ane/16", 18-1112-55	Flow restrictor, inlet
five I.d. one.0 mm x 120 cm	Fingertight connector i/16", eighteen-1112-55	Catamenia restrictor, outlet

Fingertight connector ane/16" Marriage fingertight female/M6 male

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Loading a Sample loop

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Loading a Super loop

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ÄKTApurifier 10 UPC and ÄKTApurifier 100 UPC with automation kits

Each number represents a separate ÄKTAdesign kit. For information regarding each component, place the cursor over the component of interest.

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Direct loading on to column

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Injection valve INV-907

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Multi-loading a Sample loop

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Tubing configuration for direct loading onto cavalcade

Tubing configuration when using sample pump P-960 for loading onto column

Capillary (max. length)	Connector	Connectedness points
1 I.d. 1.0 mm ten l cm (Sample inlet tubing)	Fingertight connector i/sixteen", xviii-1112-55	Sample valve, middle port*
Fingertight connector one/16", 18-1112-55 Union fingertight female/ M6 male person, eighteen-1112-57	Sample pump, inlet
2 I.d. 1.0 mm x l cm	Fingertight connector 1/16", 18-1112-55	Sample pump, outlet
Fingertight connector 1/16", 18-1112-55	Injection valve, port ii
3 PEEK i.d. 0.75 mm x fifteen cm	Fingertight connector 1/16", 18-1112-55	Injection valve, port three
Fingertight connector ane/16", 18-1112-55	Injection valve, port 6
iv I.d. one.0 mm x 130 cm	Fingertight connector one/16", eighteen-1112-55	Injection valve, port 5
5 I.d. one.0 mm 10 10 cm	Fingertight connector one/16", eighteen-1112-55	Injection valve, port 4
Fingertight connector 1/xvi", 18-1112-55	Menses restrictor, inlet
6 I.d. 1.0 mm x 120 cm	Fingertight connector one/xvi", 18-1112-55	Flow restrictor, outlet
7 PTFE i.d. one.6 mm x 130 cm*	Spousal relationship 5/16" female/ HPLC male person, 18-1142-08, Tubing connector for 1/8" o.d. tubing, xviii-1121-17 with ferrule for 1/8" o.d. tubing, 18-1121-18	Sample valve, ports 1-8*

*But if you take a sample valve in your configuration.
Note! Connect end plugs to non-used ports in the sample valve.
Note! If low sample flows are used (£10 ml/min), sample inlet tubings connected to ports ane–7
can be replaced with i.d. one.0 mm tubing together with finger-tight connectors. The sample
inlet tubing should exist adjusted to a suitable length.

Fingertight connector 1/16" Union fingertight female/M6 male person Union 5/16" female/HPLC male Tubing connector for 1/8" o.d. tubing with ferrule for 1/8" o.d. tubing.

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Sample injection using Autosampler A-900 (optional)

one. Flushed loop injection
With flushed loop injection means that the sample loop is completely filled with sample.

two. Partial loop injection
With partial loop injection means that the loop is partially filled with sample.

3. ul Pick-up injection
With ul Choice-up injection means that after aspiration from the vial, the sample volume
is transported into the loop with transport liquid (mobile stage) from some other vial.

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Accessories

Organisation accessories

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#	Product Name	Product Code	Price
5	AD-900 Analog/Digital Converter	18114862	1 756.00 USD	Add to cart Become Quote
half dozen	Sample loop, x µl	18112039	67.94 USD	Add together to cart Get Quote
7	Sample loop 100 µl	18111398	157.xiii USD	Add to cart Get Quote
8	Sample loop 500 µl	18111399	204.00 USD	Add to cart Go Quote
9	Sample loop 1 ml	18111401	171.12 USD	Add to cart Go Quote
10	Sample Loop two.0 ml, INV-907	18111402	255.00 USD	Add to cart Go Quote
eleven	Sample loop, FEP 10 ml	18116124	174.29 USD	Add together to cart Get Quote
12	Superloop, i/16" fittings, 10 ml	18111381	981.00 USD	Add to cart Get Quote
13	Superloop, one/16" fittings (ÄKTAdesign), 50 ml	18111382	1 139.00 USD	Add together to cart Get Quote
14	Superloop Consummate, 150 ml, M6 fitting	18102385	1 358.00 USD	Add together to cart Become Quote
15	Holder for XK l column	18309460	305.08 USD	Add to cart Get Quote
16	Brusk column holder, for columns 10 to 50 mm o.d.	18111317	178.48 USD	Add to cart Get Quote
17	Long Column Holder, columns 26 mm i.d.	18112632	263.00 USD	Add to cart Get Quote
18	Small Column Clamp	18114998	40.98 USD	Add to cart Get Quote
nineteen	ÄKTA Extension Equipment Holder	18115831	364.00 USD	Add to cart Get Quote
21	Electrical conductivity Flow Cell, one/16"	18111105	1 967.00 USD	Add to cart Go Quote
22	Monitor pH/C-900	18110776	nine 598.00 USD	Add to cart Get Quote
23	pH Electrode with Cell and Holder, circular tip	18113484	2 280.00 USD	Add to cart Go Quote
24	Catamenia Diversion Valve, FV-923	11001170	i 144.00 USD	Add to cart Get Quote

Software

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#	Production Proper name	Product Code	Price
	UNICORN 6.4 Workstation for ÄKTA pure, ÄKTA avant, ÄKTAexplorer, ÄKTApurifier, ÄKTApilot, ÄKTAprocess, UniFlux, and ReadyToProcess WAVE 25. (1) (2)	29056102	3 162.00 USD	Add to cart Get Quote
	UNICORN half dozen.4 Remote license without DVD (1) (2)	29067044	1 909.00 USD	Add together to cart Go Quote
	UNICORN six.4 Dry license without DVD (1) (2)	29067045	951.00 USD	Add to cart Get Quote
	UNICORN 6.4 DoE concurrent license (ane) (ii)	29067046	2 529.00 USD	Add to cart Become Quote
	UNICORN 6.four Column logbook, network license (1) (2)	29067047	1 265.00 USD	Add to cart Get Quote
	UNICORN 6.4 Remote Upgrade (ane) (2)	29067056	1 265.00 USD	Add together to cart Go Quote

ÄKTAdesign kits

Each number represents a carve up ÄKTAdesign kit.

Boosted: an ÄKTAdesign XT kit required to connect Autosampler A-900 or A-905 to the system is available, lawmaking no 18-6083-nineteen.

For information regarding each ÄKTAdesign kit, place the cursor over the number.

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Connect to BPG 100, BPG 140

For connection between a BPG 100 or BPG 140 column and ÄKTApurifier you need the post-obit parts.

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Troubleshooting

Detect solutions to product related issues. For unlisted issues please contact local Cytiva service representation.

Pressure curve

Issues related to Pressure bend

Connection to UNICORN

Issues related to UNICORN™ v.ii

pH curve

Issues related to pH bend

UV bend

Issues related to UV bend

Pump P-900 unusual appearance

Bug related to Pump P-900 unusual appearance

Valve IV-908, PV-908 or INV-907 unusual advent

Issues related to Iv-908, PV-908 or INV-907 unusual appearance

Valve SV-903 unusual appearance

Issues related to Valve SV-903 unusual appearance

General communication to achieve expert performance

Earlier using the system make sure that:

• The correct system has been selected in UNICORN System Control
• The correct wavelength has been prepare for UV/UPC monitor
• All tubing has been properly connected
• All connectors are gratuitous from leakage
• No tubing is folded or twisted
• Online filter, if used, is inverse on a regular basis
• Correct buffers are used for the chosen columns and proteins
• All inlet tubing has been immersed in correct buffer solutions
• Enough buffer has been prepared
• Buffers have been equilibrated to the environment temperature
• Buffers/eluents accept been degassed if necessary (e.g., in RPC runs)
• Suitable columns have been selected for the target proteins
• Columns have been cleaned and prepared co-ordinate to column instructions
• Samples have been clarified by centrifugation and/or filtration prior to sample loading
• Samples have been adjusted to binding buffer conditions
• Auto sampler (if used) has been prepared according to user transmission
• The fraction collector has been filled with appropriate number of microtiter plates or tubes
• Appropriate arrangement for waste handling has been prepared

Sample pump P-960 unusual appearance

Issues related to Sample pump P-960 unusual apperance

Mixer unusual appearance

Issues related to Mixer unusual advent

Conductivity curve

Issues related to Conductivity curve

Monitor UPC-900 unusual appearance

Problems related to Monitor UPC-900 unusual advent

Source: https://www.cytivalifesciences.com/en/us/support/products/aktapurifier-upc-100-28406271

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